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In several scenarios, baseline separation in the peaks might be obtained only with gradient elution and very low column loadings. So, two downsides to elution method chromatography, Specifically on the preparative scale, are operational complexity, resulting from gradient solvent pumping, and very low throughput, on account of small column loadings
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Altogether, these conclusions are more significant on condition that the endogenous amounts of L-DOPA are often taken being a measure of TH activity and which the latter is increased within the Mind of ?2A and ?2CKO mice. In fact, better L-DOPA tissue concentrations during the adrenal medulla of ?2CKO mice are used as an indication of increased TH
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